DNA

Part:BBa_K1671000:Design

Designed by: Jingcheng Shi   Group: iGEM15_Hangzhou-H14Z   (2015-09-01)


OAH


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 103
    Illegal BamHI site found at 626
    Illegal XhoI site found at 241
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 766


Design Notes

The gene sequence of this target gene contains many inappropriate enzyme cutting site, we can only constructing other cutting site with PCR by ourself.

Source

We found this sequence in the essay 'Structure of Oxalacetate Acetylhydrolase, a Virulence Factor of the Chestnut Blight Fungus.' Its accession number in GenBank is GU932672.

References